http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2096464-C1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_c345465d50afa04b4b45c50b58094b50 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K35-34 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-80 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-00 |
| filingDate | 1994-09-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 1997-11-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c859b6ac00772e768292e0956f301665 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2ecec10163f0ce847bf94eeb75c09a1b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fb815383b98d6417494753ba7152e80d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_83521abdac1ef80a1afec76a1478f09a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4d86103d10af283b260e10da94bc885c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_15e6abd5f45eca67e652036c8744fc36 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_89103e08bf17e8e1ba6a7e4a40967729 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c51ec0ffda9f57929f011bcb5a028255 |
| publicationDate | 1997-11-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | RU-2096464-C1 |
| titleOfInvention | Method of cytochrome c preparing |
| abstract | FIELD: biochemistry. SUBSTANCE: invention relates to isolation of cytochrome c from the cattle hearts. Vessels and ligaments were removed, heart tissue is defatted, milled and blood is washed out. The obtained homogenate is extracted with an aqueous solution of 0.10-0.15% sulfuric acid or 2.0-2.5% trichloroacetic acid at 10-15 C, at pH = 3.9-4.2. Extract is alkalinized to pH = 6.8-7.4 and sorption of the end product is carried out on carboxyl-cationite that is a polymer of an order that includes copolymer of methacrylic acid with N,N'-hexamethylenedimethacrylamide and copolymer of acrylic acid with divinyl benzene. Cationite is used in Na + -form. Elution is carried out with 10-12% ammonium sulfate solution at pH = 9.0-9.8. Inert proteins were precipitated from eluate by addition of ammonium sulfate at amount 0.5 g/ml elute at pH = 7.2-7.5. Cytochrome c is precipitated from supernatant with 20% trichloroacetic acid solution at cooling. Precipitate is dissolved in small water volume and dialyzed against isotonic solution of sodium chloride up to complete ions SO 2- 4 removing. EFFECT: improved method of preparing. 3 cl, 4 tbl |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2528061-C1 |
| priorityDate | 1994-09-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 43.