http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2055594-C1

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classificationIPCInventive http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K39-15
filingDate 1992-12-24-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 1996-03-10-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_76d8a62a12738abfc2147670d0fbcd04
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_40684c84716f269f65393c3ddd7976e5
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publicationDate 1996-03-10-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber RU-2055594-C1
titleOfInvention Method of preparing african equine plague virus antigen
abstract FIELD: virology. SUBSTANCE: african equine plague virus is grown in cell culture or organ animal tissue. Virus-containing suspension is frozen once at -40 C, thawed out at the room temperature and cleared by centrifugation at 3000 rev/min for 20 min. Supernatant is controlled for activity and specificity for reaction of long boudiny of complement. Then virus is inactivated by methylenebismorpholine addition at the final concentration 0.1-0.2% for 48-72 hr at 36-38 C at periodic stirring. After inactivation antigen with titer in reaction of long boudiny of complement 1:8 is concentrated with 4% polyethylene glycol of molecular mass 6000-20000 Da by 35-100 fold and used for donor immunization of specific sera or as diagnostic antigen after preserving with sodium azide 1:5000 or lyophilic drying. EFFECT: improved method of antigen preparing. 3 tbl
priorityDate 1992-12-24-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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