http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2015109081-A
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-35 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2330-51 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-72 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2800-22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2510-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-62 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-461 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-04 |
| filingDate | 2015-03-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2015-07-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | RU-2015109081-A |
| titleOfInvention | PLASMID VECTOR pET-His8-TrxL-AciP1, STRAIN OF THE BACTERIA Eschrichia coli BL21 (DE3) / pET-His8-TrxL-AciP1 FOR EXPRESSION OF ANTIMICROBIC ACEPEPSIN-1 PEPTIDE AND METHOD OF SEARCH |
| abstract | 1. Plasmid vector pET-His8-TrxL-Acip1 for expression in Escherichia coli cells of the antimicrobial peptide acipensin-1 as part of the His8-TrxL-Acip1 fusion protein, consisting of two DNA fragments: - BglII / XhoI fragment with the nucleotide sequence of SEQ ID No . 2, containing the T7 RNA polymerase transcription promoter, lac-operator, ribosome binding site and the affinity tag coding region, thioredoxin and acipensin-1 protein; - BglII / XhoI fragment of plasmid pET31b (+) containing the T7 RNA polymerase transcription terminator , replication initiation site, β-lactamase gene and lac-penpeccopa (lacI) gene. 2. The bacterial strain Escherichia coli BL21 (DE3) / pET-His8-TrxL-Acip1 is a producer of the His8-TrxL-Acip1 fusion protein obtained by transforming cells of the parent strain BL21 (DE3) with the pET-His8-TrxL-Acip1 plasmid vector according to claim 1.3. A method for producing the acipensin-1 antimicrobial peptide, comprising expressing the His8-TrxL-Acip1 fusion protein in the Escherichia coli BL21 (DE3) / pET-His8-TrxL-Acip1 fusion protein according to claim 2, cell lysis, affinity purification of His8-TrxL fusion protein -Acip1 on a metal chelate carrier, cleavage of the His8-TrxL-Acip1 fusion protein with cyanogen bromide at the methionine residue introduced between the acipensin-1 and thioredoxin sequences, repeated metal chelate purification and purification of the target peptide by reverse phase HPLC. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2618850-C2 |
| priorityDate | 2015-03-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 22.