http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2014152417-A
Outgoing Links
Predicate | Object |
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classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-53 |
filingDate | 2014-12-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2016-07-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | RU-2014152417-A |
titleOfInvention | METHOD OF MULTI-ANALYTICAL IMMUNO ANALYSIS |
abstract | 1. The method of multi-analytic immunoassay, including the immobilization at the bottom of the microplate wells of the first biospecific components specific for the analytes being determined, in the form of discrete microregions placed in the given order, introducing into the wells a sample containing the desired analytes, and second biospecific components consisting of conjugates of antibodies to determined analytes with biotin and streptavidin labeled with Pt coproporphyrin, conducting a biospecific binding reaction between the first and second biospecific composites and detecting in the time resolution mode of phosphorescence emission of the label associated with the desired analytes by scanning each discrete microregion with a focused light beam, characterized in that for the simultaneous detection of a variety of narcotic and / or psychotropic substances in the blood and / or urine of the patient as the first biospecific of components use solutions of conjugates of benzoylegonin, tetrahydrocannabinol, amphetamine, morphine, fentanyl, methamphetamine, methylenedioxytamphetamine, methadone , benzodiazepine, barbiturate and cotinine with protein, which in various combinations are immobilized in the form of at least two microregions, when analyzing, multi-analyte calibration samples containing a mixture of these drug conjugates with protein in a buffer solution are introduced into part of the microplate wells, and for urine samples and blood serums use the same multi-analytic calibration samples, test samples of urine and / or blood are added to the remaining wells, a solution in the reaction buffer is added to all wells heres mix |
priorityDate | 2014-12-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 39.