http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2014149813-A
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C40B20-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C40B20-08 |
| filingDate | 2014-12-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2016-07-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | RU-2014149813-A |
| titleOfInvention | METHOD FOR NANOPORE-PAGE DNA SEQUENCING |
| abstract | 1. A method of nanopore DNA sequencing, comprising passing a single-stranded molecule through a nanometer-sized pore in the membrane and measuring the electrical conductive properties of the pore while passing through the pore nucleotides of the chain, characterized in that the nucleic acid molecules are pre-amplified (for example, by polymerase chain reaction), divided into samples, each sample is mixed with one of the samples, each of which contains a unique variant of the single-stranded oligonucleotide molecule, the samples are labeled with the used oligonucleot to distinguish them, hybridize between DNA and oligonucleotides in the samples, for example, by heating the solution until the DNA melts and then cool, then single-stranded DNA molecules hybridized with oligonucleotides are sequenced, for which DNA molecules are passed through pores in the membrane, for example, by electrophoretic transfer molecules under the influence of an electric field and using measurements determine the fact of passage through the pore of the nucleotide single-stranded portion of the molecule and the fact of passage through the pore of the spa The nucleotide of a double-stranded portion of the molecule, for example, measures the change in the current strength through the pore, thereby determining and distinguishing cases of the passage of the nucleotide on the single-stranded molecule and the passage of the double-stranded portion of the molecule or use pores of this size through which only a single-stranded DNA molecule can pass, in this case, the speed of the molecule is recorded and the entry into the pore of the double-stranded region is determined by slowing the movement of the molecule through the pore or by stopping the movement of lekul |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11567060-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2747714-C1 |
| priorityDate | 2014-12-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 17.