http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2014144722-A
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6851 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-689 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6888 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-689 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6851 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6853 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6844 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6848 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6806 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6895 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-701 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 2013-04-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2016-06-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | RU-2014144722-A |
| titleOfInvention | COMPOSITIONS AND METHODS FOR QUANTITATIVE DETERMINATION OF THE NUCLEIC ACID SEQUENCE IN THE SAMPLE |
| abstract | 1. A method for quantifying a specific product in an amplification reaction with single stranded breaks and completion, the method comprising: (a) bringing the target nucleic acid molecule into contact under practically isothermal conditions with a polymerase, two or more oligonucleotide primers / matrices, each of which specifically binds to a complementary sequence in the target nucleic acid molecule, a single stranded break enzyme, and a detectable polynucleotide probe, where e each of the oligonucleotide primers / templates contains one or more 2-modified nucleotides in a sequence complementary to the target nucleic acid molecule; (b) the formation of amplicons containing at least a portion of the specified target nucleic acid molecule; and (c) detecting a signal specific for hybridization of the oligonucleotide probe with the target nucleic acid molecule or its amplicon, where the signal indicates the amount of the target nucleic acid molecule present in the sample or its amplicon. 2. The method of claim 1, wherein the 2′-modification is selected from the group consisting of 2′-O-methyl, 2′-methoxyethoxy, 2′-fluoro, 2′-hydroxyl, 2-allyl, 2′-O - [2- (methylamino) -2-oxoethyl], 4′-thio, 4′-CH-O-2′-bridge, 4 ′ - (CH) -O-2′-bridge, 2′-LNA and 2 ′ -O- (N-methylcarbamate) or analogous bases. 3. A method according to claim 1, characterized in that one or more 2′-modified nucleotides are located at the 3′-end of the sequence complementary to the target nucleic acid molecule. The method of claim 1, wherein one or more 2′-modified nucleotides are located at the 5′-end of the last |
| priorityDate | 2012-04-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 28.