http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2014141207-A
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2800-367 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-56927 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-56905 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-56994 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-56988 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-56983 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-577 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-533 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-569 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-569 |
| filingDate | 2013-03-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2016-05-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | RU-2014141207-A |
| titleOfInvention | A method for detecting intracellular pathogens of infection in sperm cells |
| abstract | 1. A method for testing for the presence of viruses, chlamydia, parasites and other pathogens of infection intracellularly in spermatozoa, the method comprising the following steps: a procedure for facilitating the dense structure of sperm cell DNA, including sperm, direct and indirect immunofluorescence inside sperm cells , - visualization and evaluation of results using flow cytometry, and this method is characterized in that the stage of facilitating the dense DNA structure must necessarily occur before immunofluorescence. 2. The method of claim 1, wherein the step of visualizing and evaluating the results using flow cytometry involves incubating cell pellets with 7-aminoactinomycin D (7AAD) in WB in order to distinguish between 1N and 2N.3 cells. The method according to any one of paragraphs. 1 or 2, which is used to determine the causes of infertility, early pregnancy termination, miscarriage or fetal death and for the prevention of congenital infections or for the prevention of vertical transmission, as well as for the study of inflammation and infections of the male reproductive system. 4. The method according to claim 1, which is used specifically for the study of one of the following pathogens: cytomegalovirus (CMV), herpes simplex virus I (HSV I), herpes simplex virus II (HSV II), Epstein-Barr virus (EBV), HHV6, HHV7, HHV8, parvovirus 19, hepatitis B virus (HBV), hepatitis C virus (HCV), coxsacki virus, human immunodeficiency viruses (HIV-1, HIV-2), adeno-associated virus (AAV), rubella virus, HPV, chlamydia , toxoplasma and norovirus. 5. The method according to claim 1, which is used to study the presence of chlamydia in spermatozoa in combination with a spermogram and culture using the same sample, or on different samples. The method of claim 1, wherein the relief is very |
| priorityDate | 2012-03-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 73.