http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2014139329-A
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K39-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61P31-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-53 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K39-40 |
| filingDate | 2014-09-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2016-04-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | RU-2014139329-A |
| titleOfInvention | METHOD FOR PRODUCING BRUCELLA ABORTUS 19 STRAIN CELL CONCENTRATE OF BRUCELLA ABORTUS 19 STRAIN FOR PREPARATION OF BRUCELLOS ANTIGENES (THREE OPTIONS), METHOD FOR PRODUCING BRUSSULOSELA BEROSELO |
| abstract | 1. A method of obtaining a brucella cell culture concentrate from Brucella abortus 19 strain for the preparation of brucellosis antigens used in diagnostic test systems, the method comprising obtaining inoculum of bacterial cells and culturing them under deep conditions in a liquid nutrient medium with regulation of the level of oxygen concentration dissolved in the culture medium throughout the cultivation process, inactivation of the grown bacterial cells by heating, followed by their concentration, the process ultivization of bacterial cells is carried out at a temperature of 36-38 ° C, during cultivation at the stage of accumulation of bacterial cells from a concentration of 1.5-2.5 billion MK./cm to a concentration of 10 billion MK./cm maintain the concentration of dissolved oxygen in culture medium in the range of 20-25 mg / l, and more than 10 billion MK./media until the bacterial cells reach the stationary growth phase, they provide the concentration of dissolved oxygen in the culture medium in the range of 40-45 mg / l, and the concentration of the grown bacterial cells is infused by precipitation with a flocculant, which is used as sodium salt of carboxymethyl cellulose (CMC), which is added to the culture medium in an amount of 0.1-0.15 g dry matter per 1 liter of culture medium, and then the supernatant is drained to obtain a concentrate of inactivated bacterial cells for the subsequent preparation of brucellosis antigen in RA, RSK, RDSK, RBP antigen; KR antigen with milk. 2. The method according to claim 1, characterized in that the deposition is carried out for 24-48 hours at a temperature of from 8 ° C to 12 ° C. 3. The method according to claim 1, characterized in that the cool |
| priorityDate | 2014-09-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 45.