http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2014127326-A
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y207-02007 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y103-99002 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y402-01017 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y602-01002 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-93 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P7-42 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-88 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-13 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-001 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-52 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-1217 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-0004 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P7-42 |
| filingDate | 2012-11-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2016-02-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | RU-2014127326-A |
| titleOfInvention | Biotechnological production of 3-hydroxyisobutyric acid |
| abstract | 1. A method comprising the following steps: a) providing isobutyric acid, b) contacting isobutyric acid with a combination of isobutyrate kinase and phosphotransisobutyrylase and / or isobutyryl-Coenzyme A synthase / ligase and / or isobutyrate-Coenzyme A transferase, c) administration the product from step b) into contact with isobutyryl-Coenzyme A-dehydrogenase, d) introducing the product from step c) into contact with methacryl-Coenzyme A-hydrate, e) hydrolysis of the product from step d) to form 3-hydroxyisobutyric acid, where at least one of the enzymes used in stages b), c) and d) from the group of isobutyrate kinase, phosphotransisobutyrylase, isobutyryl-coenzyme A synthase / ligase and isobutyrate-coenzyme A-transferase, preferably all, are provided in the form of a cell which, compared to its wild type, has a reduced activity of 3-hydroxyisobutyric acid dehydrogenase or a variant thereof. 2. A method according to claim 1, wherein isobutyric acid is formed by contacting isobutane with a monooxygenase, preferably an alkane hydroxylase, more preferably an AlkBGT type or a variant thereof. The method of claim 1, wherein the hydrolysis in step d) is achieved by contacting the product from step d) with 3-hydroxyisobutyryl-Coenzyme A-hydrolase. The method of claim 1, wherein the cell contains both isobutyryl-coenzyme A dehydrogenase from step c) and methacrylyl-coenzyme A hydratase from step d), as well as a combination of isobutyrate kinase and phosphotransisobutyrylase and / or isobutyryl-coenzyme A synth ligase and / or isobutyrate-coenzyme A transferase. 5. The method of claim 1, wherein the cell further comprises an alkane hydroxylase, preferably an AlkBGT-type or variant thereof. The method of claim 1, wherein 3-hydroxyisobutyric acid dehydrogenase is |
| priorityDate | 2011-12-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 51.