http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2014116060-A
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-53 |
| filingDate | 2014-04-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2015-10-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | RU-2014116060-A |
| titleOfInvention | METHOD FOR DIAGNOSTIC IN VITRO OF IMPROVED SENSITIVITY TO PSEUDOALLERGENES AND SELECTION OF ANTI-ALLERGIC DRUGS |
| abstract | 1. A method for the in vitro diagnosis of hypersensitivity to pseudo-allergens and the selection of antiallergic drugs, which consists in the selection and preparation of venous blood, the administration of the test drug and the analysis of luminol-dependent chemiluminescence (CL), characterized in that, in parallel with luminol-dependent CL, lucigenin-dependent analysis is carried out CL, for which an anticoagulant is injected into the selected blood, samples containing 0.95-1.05 × 10 leukocytes are formed, and adjusted with Hanks solution to a volume of 0.69 ml to obtain blood samples, the test drug a sevdoallergen or test preparation of pseudo-allergen in a mixture with an antiallergic drug, and a control preparation of 0.01 ml are injected into the obtained blood samples and incubated with constant stirring for 45 min at 37 ° C, then luminol and lucigenin activators are separately injected after the incubated samples in the amount of 2 mM and a chemiluminometer measure the level of spontaneous CL for the indicated activators, after which the luminescent stimulator - barium sulfate in the amount of 2 mg / ml is introduced into the same samples and the level of stimulated CL is also for these activators, and CL measurements are carried out in constant mixing mode at a temperature of 37 ° C for 45 min to obtain time dependences of the CL level, and hypersensitivity to pseudo-allergens is judged by the IP index, determined by the expression IP = Spa / Sк, where is Spa; Sk - the area under the curves of the time dependence of the level of spontaneous and stimulated CL for the tested and control drugs, respectively. 2. The method according to p. 1, characterized in that the test drug sodium salicylate is bred in physiological |
| priorityDate | 2014-04-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 34.