http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2014113509-A
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-20 |
| filingDate | 2014-04-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2015-10-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | RU-2014113509-A |
| titleOfInvention | METHOD FOR DIFFERENTIATION OF PLAGUANIUM ACTIVITIES AND PSEUDO-TUBERCULOSIS ON N-ACETYL-β-D-GLUCOSAMINIDASE ACTIVITY |
| abstract | The method for differentiating causative agents of plague and pseudotuberculosis by N-acetyl-β-D-glucosaminidase activity includes the following stages: a) a suspension of the agar culture of the studied bacteria is obtained in a concentration (1-5 × 10 m.k.); b) a synthetic substrate is prepared, for this in its quality, take a substrate of 4-methylumbelliferyl-N-acetyl-β-D-glucosaminide in an amount of 50 μM, which is dissolved in 2 ml of dimethylformamide, then 0.6 ml is taken from the resulting solution and 9.4 ml of 0.1 are added to it M phosphate buffer pH 7.4; c) the prepared substrate in an amount of 20 μL mixed with a drop (0.05 ml) of a suspension of bacterial agar culture in physiological solution placed in a Petri dish; g) incubate the resulting mixture at 37 ° C for 10-20 minutes, after which the reaction is stopped and 5 μL 10 is added to it N alkali solution; e) differentiation is carried out in the UV rays of the transilluminator at 366 nm, bright blue fluorescence indicates a positive result of the reaction, which exhibits N-acetyl-β-D-glucosaminidase activity and confirms that the studied strain hydrolyzes prepared with the product, therefore, it is identified as a strain of Yersinia pseudotuberculosis, and the absence of luminescence confirms its belonging to strains of Yersinia pestis. |
| priorityDate | 2014-04-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 21.