http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2014108901-A
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y302-01021 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-12 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-2437 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y302-01091 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y302-01004 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-62 |
| filingDate | 2014-03-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2015-09-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | RU-2014108901-A |
| titleOfInvention | WHOLE CELL BIO-CATALYSTS IN THE DEGRADATION OF CELLULAR BIOMASS |
| abstract | 1. A nucleic acid molecule containing the following components: (1) a signal peptide-coding region, (2) a heterologous cellulase-coding region, (3) an optional protease-recognition site, (4) a transmembrane linker-coding region, and ( 5) a region encoding the transport domain of an autotransporter protein or its variant. 2. A nucleic acid molecule according to claim 1, characterized in that the cellulase is beta-glucosidase or an endo- or exocellulase, preferably selected from the group consisting of Bacillus subtilis endocellulase, Clostridium thermocellum exocellulase and Clostridium thermocellum beta-glucosidase. 3. A nucleic acid molecule according to claim 1, characterized in that the transport domain of the autotransporter protein is selected from the group consisting of Ssp, Ssp-h1, Ssp-h2, PspA, PspB, Ssa1, SphB1, AspA / NalP, VacA, AIDA-I, IcsA, MisL, TibA, Ag43, ShdA, AutA, Tsh, SepA, EspC, EspP, Pet, Pic, SigA, Sat, Vat, EpeA, EatA, EspI, EaaA, EaaC, Pertactin, BrkA, Tef, Vag8, PmpD, Pmp20, Pmp21, AgA1 protease, App, Hap, rOmpA, rOmpB, ApeE, EstA, Lip-1, McaP, BabA, SabA, AlpA, Aae, NanB or their variants. 4. A nucleic acid molecule according to any one of paragraphs. 1-3, characterized in that it contains an expression control sequence and is operably linked to a nucleic acid molecule, which can preferably be activated by the addition of isopropylthioglucopyranoside (IPTG) or arabinose. 5. The polypeptide encoded by the nucleic acid according to any one of paragraphs. 1-4 claims 6. A microorganism expressing a polypeptide according to claim 5 on the surface or transformed using a nucleic acid molecule according to any one of claims. 1-4.7. The microorganism according to claim 6, characterized in that it is based on a gram-negative microorganism, preferably Escherichia coli. Membrane fraction, � |
| priorityDate | 2013-03-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 89.