http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2013137272-A
Outgoing Links
Predicate | Object |
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classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-07 |
filingDate | 2013-08-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2015-02-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | RU-2013137272-A |
titleOfInvention | METHOD FOR PRODUCING AND INDUCTION OF DIRECTED DIFFERENTIATION OF CULTURE OF MULTIPOTENT LUNG CELLS |
abstract | A method for producing and inducing directed differentiation of a culture of multipotent lung cells, characterized in that populations of cells are isolated from the tissue of fibrosed lungs, cultured for 60 days, passaged twice, a culture of adhesion cells of fibrosed lungs, morphologically representing round cells when transformed into spindle-shaped cells, is obtained that express CD44, CD73, CD90, CD106 markers on the surface, do not express CD31, CD34, CD45, then in the presence of growth factors These mononuclear cells differentiate in vitro into adipocytes (60 μM indomethacin, 1 μg / ml insulin), chondrocytes (10 ng / ml recombinant human TGF-β1), osteoblasts (10 mM β-glycerophosphate), fibroblasts (2 μg / ml fibroblast growth factor ) |
priorityDate | 2013-08-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 94.