http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2013133626-A
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-245 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-1029 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N1-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P13-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-74 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-52 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-11 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y203-01031 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-245 |
| filingDate | 2011-12-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2015-01-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | RU-2013133626-A |
| titleOfInvention | MODIFIED POLYPEPTIDE POSSESSING HOMOSERINACETHYL TRANSFERASE ACTIVITY AND EXPRESSING ITS MICROORGANISM |
| abstract | 1. Modified polypeptide with homoserine-O-acetyltransferase activity with amino acid sequence SEQ ID NO: 17 or with at least 95% homology with it, in which the amino acid at position 111 from the initial amino acid methionine of the sequence is replaced with glutamic acid. The modified polypeptide of claim 1, wherein the amino acid at position 112 of the polypeptide is additionally replaced with threonine or histidine. The modified polypeptide according to claim 1 or 2, wherein the modified polypeptide has any of the amino acid sequences of SEQ ID NO: 18-20.4. The modified polypeptide of claim 1, wherein the modified polypeptide exhibits resistance to methionine feedback regulation upon amino acid substitution. The modified polypeptide of claim 4, wherein the amino acid at position 29 is replaced with proline, at position 114 is replaced with glycine, at position 140 is replaced with serine, or one or more combinations thereof. The modified polypeptide of claim 5, wherein the amino acid at position 112 of the polypeptide is additionally replaced with threonine or histidine. The modified polypeptide according to claim 5 or 6, wherein the modified polypeptide has any of the amino acid sequences of SEQ ID NO: 21-23.8. A polynucleotide encoding a modified polypeptide according to clause 1.9. The polynucleotide of claim 8, wherein the polynucleotide has any of the nucleotide sequences of SEQ ID NO: 24-29. A recombinant vector containing polynucleotide sequences operably linked to a polynucleotide according to clause 8.11. A microorganism containing a polynucleotide according to clause 8.12. The microorganism according to claim 11, where the microorganism is additionally modified to have increased acetyl-CoA synthetase. |
| priorityDate | 2010-12-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
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