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filingDate 2011-11-30-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationDate 2015-01-10-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber RU-2013129990-A
titleOfInvention CELL LINES THAT SECRET THE STRUCTURES OF ANTI-ANGIOGENIC ANTIBODY-FRAME AND SOLUBLE RECEPTORS AND THEIR APPLICATION
abstract 1. A cell line containing ARPE-19 cells genetically engineered to produce a therapeutically effective amount of one or more anti-angiogenic antibody-scaffold constructs or anti-angiogenic molecules, wherein said one or more anti-angiogenic antibody-scaffold constructs or anti-angiogenic molecules are introduced into cells ARPE-19 using an iterative transfection method, wherein said iterative transfection comprises one transfection, two transfection, or three transfections. 2. A cell line according to claim 1, wherein said cell line produces one or more anti-angiogenic antibody-scaffold constructs or anti-angiogenic molecules in an amount of 10,000-30000 ng / day / 10 cells, if one transfection is one transfection. 3. A cell line according to claim 2, wherein said cell line produces one or more anti-angiogenic antibody-scaffold constructs or anti-angiogenic molecules in an amount of 15,000 ng / day / 10 cells. A cell line according to claim 1, wherein said cell line produces one or more anti-angiogenic antibody-scaffold constructs or anti-angiogenic molecules in an amount of 30000-50000 ng / day / 10 cells, if two transfections are iterative transfection. The cell line according to claim 4, wherein said cell line produces one or more anti-angiogenic antibody-scaffold constructs or anti-angiogenic molecules in an amount of 35,000 ng / day / 10 cells. A cell line according to claim 1, wherein said cell line produces one or more anti-angiogenic antibody-framework constructs or anti-angiogenic molecules in an amount of 50,000-75,000 ng / day / 10 cells, if iterative transfection is tr
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