http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2013124809-A
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-535 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-36 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-27 |
| filingDate | 2011-10-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2014-12-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | RU-2013124809-A |
| titleOfInvention | METHOD FOR CLEANING A HUMAN FACTOR STIMULATING A COLONY OF GRANULOCYTES FROM RECOMBINANT E. coli |
| abstract | 1. A method for purifying human factors stimulating granulocyte colonies (hG-CSF) from recombinant E. coli in high yield, comprising the steps of: (a) cultivating hG-CSF-expressing recombinant E. coli to obtain a cell pellet by centrifugation; (b ) separating the hG-CSF-containing supernatant from the cell pellet obtained in step (a); (c) treating the supernatant obtained in step (b) with an acid to separate the resulting pellet by filtration; (d) applying to the filtrate obtained in the stage and (c) cation exchange chromatography; (e) applying hydrophobic interaction chromatography to the eluate obtained in step (d); and (f) applying to the eluate obtained in step (e), anion exchange chromatography. 2. The method of claim 1, wherein in step (a), hG-CSFs are expressed in the periplasmic space of recombinant E. coli. The method of claim 2, wherein the recombinant E. coli is one or more bacterial species selected from the group consisting of E. coli BL21 (DE3) / pT14SS1SG (HM 10310), E. coli BL21 (DE3) / pT14SS1S17SEG (HM 10311; registration No. KCCM-10154), E. coli BL21 (DE3) / pTO1SG (HM 10409), E. coli BL21 (DE3) / pTO1S17SG (HM 10410; registration No. KCCM-10151), E. coli BL21 ( DE3) / pTO17SG (HM 10411; Registration No. KCCM-10152), E. coli BL21 (DE3) / pTO17TG (HM 10413), E. coli BL21 (DE3) / pTO17AG (HM 10414), E. coli BL21 (DE3 ) / pTO17GG (HM 10415), E. coli BL21 (DE3) / pBAD2M3VG (HM 10510; registration No. KCCM-10153), E. coli BL21 (DE3) / pBAD17SG (HM 10511) and E. coli BL21 (DE3) / pBAD2M3V17SG (HM 10512) .4. The method of claim 1, wherein in step (b), the hG-CSF-containing supernatant is separated from the cell pellet by osmotic extraction. The method according to claim 4, wherein the osmotic extraction is carried out by treating the cell pellet with a buffer solution containing 10-30% sucrose to obtain a cell pellet |
| priorityDate | 2010-10-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 38.