http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2013124434-A
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 2013-05-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2014-12-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | RU-2013124434-A |
| titleOfInvention | METHOD FOR QUANTITATIVE DETERMINATION OF SPECIES COMPOSITION OF PROPION BACTERIA INHABITING HUMAN SKIN |
| abstract | A method for quantitative determination of the species composition of propionic bacteria living on human skin, which consists in the fact that DNA is isolated from a sample obtained by grinding a skin area with a sterile cotton swab dipped in alkaline phosphate buffer using an EX-509 DNA extran test kit, 100 μl of the suspension bacterial cells are placed in plastic tubes, before the start of isolation, to control the degree of RNA output, 5 × 104 copies of the Ptgt 2 plasmid are added to the samples in 5 μl of 10 mM Tris-HCl buffer (pH 8.0), the cells are lysed with 300 μl 1% SDS solution containing 20 mM Tris-HCl buffer (pH 8.0), 75 mM NaCl and 25 mM Na2EDTA for 10 min at room temperature, proteins were precipitated with 100 μl of 7.5 M NH4 (CH3COO) followed by centrifugation of 12000g for 2 min, the supernatant is transferred into plastic tubes, the DNA is precipitated with an equal volume of isopropanol at room temperature, centrifuged at 12000g for 2 minutes, the DNA pellet is washed with 75% ethanol and dried in test tubes in air at room temperature until the alcohol evaporates completely, the DNA is dissolved in 100 μl of 10 mm Tris-HCl buffer (pH 8.0) at 65 ° C for 5 min, and then conduct a quantitative PNR reaction with primers GCG TGA GTG ACG GTA ATG GGT A, TTC CGA CGC GAT CAA CCA, FAM-AGC GTT GTC CGG ATT TAT TGG ATC CGG GAG CTT C, ACCA CA CAT STC ACG ACA CG, R6G-CGG TTC ACA GGT GGT GCA TTG GC-BHQ2, GTC TGC AAC TCG ACC CCA T, AGT CCT AAT TAC CAG TCC CAC, ROX-ACC TGT GTG GGG GAG CCG TCG AAG-BHQ2, AGA ACT GCT AGC TGT AG, CGT AGA ACT AGC TGT AGC GCA, Cy5-AG CGG STS STA CTT CTG CAG GGG-BHQ2. |
| priorityDate | 2013-05-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 20.