http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2012153785-A
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-2302 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-2301 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-20 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-2321 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-60 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-231 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2015-149 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2015-008 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2502-1157 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-72 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-24 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-25 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2502-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2502-1114 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2502-1185 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-30 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-32 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-50 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-533 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-53 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N15-14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-577 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0635 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-50 |
| filingDate | 2011-05-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2014-07-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | RU-2012153785-A |
| titleOfInvention | METHOD FOR CULTIVATING A SINGLE B-CELL |
| abstract | 1. A method for selecting B cells, comprising the following steps: a) co-culturing each B cell from a population of B cells that were stored as single cells with feeder cells, b) selecting a B cell clone that proliferates and secrets the antibody on stage a) .2. A method for producing an antibody that binds to a target antigen, comprising the following steps: a) co-culturing each B cell from a population of B cells that were stored as single cells in separate containers in the presence of feeder cells and a feeder mixture, b) selection B a cell clone producing an antibody that specifically binds to a target antigen, c) culturing a cell that contains a nucleic acid that encodes an antibody that is produced by the B cell clone selected in step b), or a manipulated variant, and isolating the antibody from the cell or culture supernatant and thereby producing the antibody. 3. The method according to claim 1 or 2, further comprising the step of incubating the B-cell population in a co-culture medium before storing a single cell. The method according to claim 3, characterized in that the incubation is carried out at a temperature of 37 ° C for about one hour. The method of claim 1 or 2, further comprising the step of centrifuging the single stored B cells before co-culture. The method according to claim 5, characterized in that the centrifugation is carried out for 5 minutes at a speed of about 300 g. A method according to claim 1 or 2, characterized in that the population of B cells is isolated by centrifugation in a density gradient. The method according to claim 1 or 2, which |
| priorityDate | 2010-05-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 184.