http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2012143314-A
Outgoing Links
Predicate | Object |
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classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K31-00 |
filingDate | 2012-10-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2014-04-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | RU-2012143314-A |
titleOfInvention | METHOD FOR OBTAINING AN ANTIVIRAL AGENT AND AN ANTIVIRAL AGENT |
abstract | 1. A method of obtaining an antiviral agent by preparing the seed mycelium of basidiomycete winter mushroom Flammulina velutipes (Curtis) Singer, preparing a liquid nutrient medium containing water, vegetable oil in the amount of 19.0-27.0 g / l water and molasses as carbon sources in an amount of 15-40 g / l of water, as a source of nitrogen - corn flour in an amount of 7-15 g / l of water, as mineral salts of potassium dihydrogen phosphate in an amount of 2.0-2.4 g / l of water and magnesium sulfate in the amount of 0.18-0.20 g / l of water, its sterilization, sowing prepared sowing the mycelium of a sterile liquid nutrient medium, culturing in it basidiomycete under aerobic conditions to obtain an immersed culture, followed by separation of the immersed culture into the biomass of basidiomycete and the culture fluid and isolating an antiviral agent from it. 2. The method according to claim 1, characterized in that the liquid nutrient medium additionally contains zeatin in an amount of 0.0010-0.0015 g / l of water, vitamin B3 in an amount of 0.0060-0.0065 g / l of water, yeast extract in an amount 0.050-0.055 g / l of water. 3. The method according to claim 1, characterized in that the antiviral agent is isolated from the culture fluid by precipitating it with 96% ethanol at a temperature of 4-6 ° C, with a volume ratio of the culture fluid and ethyl alcohol of 1: 2 - 1: 4.4. An antiviral agent prepared according to any one of claims 1-3. |
priorityDate | 2012-10-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 27.