http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2012137428-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_408ea6b9888bfe45e8cb84b828d1ea24 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-56 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N1-38 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-38 |
| filingDate | 2011-01-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3c88f0768c83241b798c2a7263921ae2 |
| publicationDate | 2014-03-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | RU-2012137428-A |
| titleOfInvention | METHOD FOR PRODUCING ALPHA5-INTERFERON PROTEIN |
| abstract | 1. A method for producing alpha5-interferon (IFNa5) protein by expression in an Escherichia coli (E. coli) host cell, including using an E. coli host cell producing IFNa5, cultivating an E. coli host cell producing IFNa5 under conditions effective for expression of IFNa5 by an E. coli host cell, expression of IFNa5 by an E. coli host cell via a fermentation medium fed with a carbon feed solution, isolation and optional purification of the expressed IFNa5 protein, wherein the carbon feed solution contains a carbon source and per liter the following components, mg: and the specified fermentation medium is essentially free from components of animal origin or yeast origin and is characterized by the content per liter of the following components, mg: 2. The method according to claim 1, wherein the E. coli host cell is an E. coli host cell transformed with a vector containing the IFNa5 protein coding sequence under the control of an inducible promoter. The method according to claim 1 or 2, wherein the E. coli host cell is an E. coli host cell that is a protease-deficient E. coli strain, preferably a protease-deficient E. coli lon/ompT host strain, more preferably E. coli strain BL21, most preferably E. coli strain BL21 (DE3).4. The method of claim 3, wherein the E. coli host cell of E. coli strain BL21 (DE3) is used as the E. coli host cell, and expression of IFNa5 is induced by isopropyl-β-D-thiogalactopyranoside. The method according to claim 4, which is characterized by an average specific growth rate of the culture (µ) after induction of at least 0 |
| priorityDate | 2010-02-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 39.