http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2012136589-A
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-02 |
| filingDate | 2012-08-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2014-02-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | RU-2012136589-A |
| titleOfInvention | RECOMBINANT HUMAN OREXIN USED TO EXTEND THE WAKE-UP PERIOD AND METHOD FOR PRODUCING IT |
| abstract | 1. The preparation of recombinant human orexin A, which is a sequence of 33 amino acids with a molecular weight of 3583 Da (SEQ ID NO: 1), for the treatment of diseases associated with dysfunction of orexin-containing systems, maintenance of wakefulness, and correction of eating behavior. 2. 5859 bp pG-hOrexA plasmid DNA containing an insert of the orexA gene encoding human orexin A according to claim 1, as well as the following components: a) a promoter for T7 polymerase polymerase, which provides control of gene expression level, b) lac -operator to ensure controlled expression of the gene encoding the desired protein, c) the ribosomal complex binding site (RBS), d) the start codon for the translation of the cloned fragment, e) the rop fragment provides the optimal copy number of the plasmid, e) pBR322ori for replication initiation, g ) lacI - lact zny repressor to operate the lac-operon, s) join the ampicillin resistance for selection of clones containing plazmidu.3. A method of obtaining a highly purified preparation of native recombinant human orexin A, which consists in the fact that 1) clone the cDNA of the gene encoding human orexin A, as part of plasmid DNA 2) transform cells of E. coli strain DH10B (FmcrA, Δ (wrr-hsdRMS-mcrBC) , φ80dlacZΔM 15, ΔlacX74, deoR, recA1, endA1, araD139, Δ (ara, leu) 769, galU, gaIKλ, rpsL, nupG) by electroporation 3) isolate recombinant plasmid DNA (pG-hOrexA), 4) transform coli strain BL21 to obtain recombinant protein in large quantities created by the plasmid by electroporation, 5) conduct induction Press gene encoding the protein in E.coli cells strain BL21 (genotype F-ompT hsdSB (rB-mB-) gal dcm rne131) 0.2% lactose on Study Method |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2647771-C2 |
| priorityDate | 2012-08-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 41.