http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2012130940-A
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2506-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2503-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0613 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0678 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0068 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-999 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-063 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0603 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-067 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0676 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-071 |
| filingDate | 2010-12-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2014-01-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | RU-2012130940-A |
| titleOfInvention | DIFFERENTIATION OF HUMAN EMBRYONAL STEM CELLS |
| abstract | 1. A method of increasing the expression of NGN3 and NKX6.1 in a population of cells expressing markers characteristic of the endocrine line of the pancreas, comprising the following stages: a) cultivating pluripotent stem cells, b) differentiating pluripotent stem cells into cells expressing markers characteristic of the line definitive endoderm, c) differentiating cells expressing markers characteristic of the definitive endoderm line into cells expressing markers characteristic of the pancreatic endoderm line, by adding to the medium used to differentiate the definitive endoderm line, compounds selected from the group consisting of H-9, H-89, GF 109203X, HA-1004, PP2, PP1, LY 294002, Wortmannin, SB-203580, SB- 202190, Tirfostina 25, Tirfostina, AG1478, Tirfostina 46, GW 5074, Kenpaullon, HNMPA, AG490, Y27632 and ML-7, and d) differentiation of cells expressing markers characteristic of the pancreatic endoderm line into cells expressing markers characteristic of endocrine pancreatic line. 2. The method of claim 1, wherein compounds selected from the group consisting of H-9, H-89, GF 109203X, HA-1004, PP2, PP1, LY 294002, Wortmannin are added to the medium used to differentiate the pancreatic endoderm line , SB-203580, SB-202190, Tirfostina 25, Tirfostina, AG1478, Tirfostina 46, GW 5074, Kenpaullon, HNMPA, AG490, Y27632 and ML-7. |
| priorityDate | 2009-12-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 23.