http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2012116151-A
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12R2001-01 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C01G43-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A62D3-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-195 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-705 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N1-205 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-705 |
| filingDate | 2010-09-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2013-10-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | RU-2012116151-A |
| titleOfInvention | ISSUE OF PROTEIN RESPONSIBLE FOR RESTORATION OF URANIUM (VI) |
| abstract | 1. An isolated polypeptide derived from Thermus scotoductus strain SA-01, which is responsible for the reduction of uranium (VI) in a uranium (VI) source to uranium (IV), wherein the polypeptide comprises the amino acid sequence of SEQ ID No: 1.2. The polypeptide according to claim 1, which is characterized in that it is a homogeneous protein having a molecular weight of 70 kDa, as shown by polyacrylamide gel electrophoresis with sodium dodecyl sulfate. The polypeptide of claim 1, wherein the polypeptide, as shown by NCBI BLASTP analysis, is a peptide-binding protein, an ABC peptide transporter. The polypeptide of claim 1, wherein the U (VI) source is selected from the group consisting of UO (CHCO) .2HO and UO (NO). 5. Isolated nucleic acid molecules that encode the amino acid sequence of SEQ ID No: 1, including the nucleotide sequence of SEQ ID No: 2.6. A vector comprising the nucleic acid molecules of claim 5.7. A host cell comprising the vector of claim 6.8. A method of obtaining at least one polypeptide according to any one of claims 1 to 4, which is responsible for the reduction of uranium (VI) in a source of uranium (VI) to uranium (IV), which includes the steps of: a) transfection of nucleic acid molecules SEQ ID NO: 2 into a host cell b) culturing a host cell to express the polypeptide of SEQ ID NO: 1 in a host cell; and c) optionally isolating and purifying the polypeptide of SEQ ID NO: 1.9. Microorganism transformed with the U (VI) resistance gene obtained from the host cell according to claim 7, with the sequence SEQ ID No: 1.10. A method for bio-purification or at least partial bio-purification of an area contaminated with a source of U (VI), which includes the steps of introducing an electron donor into the contaminated area in order to stimulate the proliferation of strain SA-01 Thermus scotoductus to restore U (VI) in the source |
| priorityDate | 2009-09-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 33.