http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2012114662-A
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-31 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-95 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1041 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P35-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-6845 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-525 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-6878 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-11 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-62 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1044 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-47 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1034 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-435 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1037 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K19-00 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-00 |
| filingDate | 2010-12-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2013-10-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | RU-2012114662-A |
| titleOfInvention | MODIFIED UBIQUITINE PROTEINS WITH SPECIFIC BINDING ACTIVITY FOR EXTRADOMAIN "B" FIBRONECTIN |
| abstract | 1. A protein capable of binding the extradomain B (ED-B) of fibronectin, including a modified heterodimeric ubiquitin protein, in which two monomeric ubiquitin units are connected to each other in a head-to-tail arrangement, characterized in that each monomer of said dimeric protein is different modified by substitutions of at least 6 amino acids at positions 2, 4, 6, 8, 62, 63, 64, 65, 66 and 68 of SEQ ID NO: 1, said substitutions including: (1) in the first monomeric substitution unit of at least least in amino acid positions 6, 8, 63, 64, 65 and 66; and the second monomeric substitution unit at least at amino acid positions 6, 8, 62, 63, 64, 65 and 66; optionally an additional 2, or (2) in the first monomeric substitution unit at least at amino acid positions 2, 4, 6, 62, 63, 64, 65, and 66; and the second monomeric substitution unit at least at amino acid positions 6, 8, 62, 63, 64, 65 and 66; optionally, additionally 2, and optionally other modifications, preferably substitutions of other amino acids, wherein said modified monomeric ubiquitin unit has an amino acid identity with SEQ ID NO: 1, at least one from the group of 80%, at least 85%, and at least 90%, and wherein said protein has a specific binding affinity for said fibronectin ED-B domain with kD = 10-10M and exhibits monovalent binding activity with said fibronectin extradomain B (ED-B). 2. The protein according to claim 1, characterized in that said substitutions include: (1) in the first monomer unit of at least K6W, L8W, K63R, E64K, S65F and T66P; and in the second monomer unit at least K6T, L8Q, Q62W, K63S, E64N, S65W and T66E; optionally, optionally Q2R, or (2) in the first monomer |
| priorityDate | 2009-12-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 72.