http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2012112582-A
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-00 |
| filingDate | 2012-03-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2013-10-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | RU-2012112582-A |
| titleOfInvention | ALLOCATED NUCLEIC ACID ENCODING A FLUORESCENT BIOSYCENSOR, EXPRESSION CASSETTE, CELL PRODUCING A FLUORESCENT BIOSENSOR, ALLOCATED FLUORESCENT BIOSENCER |
| abstract | 1. An isolated nucleic acid encoding a fluorescent biosensor for measuring changes in the NAD + / NADH ratio within cells, the amino acid sequence of which is shown in SEQ ID NO: 4, where the biosensor responds by increasing the signal when the NAD + / NADH ratio shifts to a decrease in the NADH concentration. 2. An expression cassette, which, when integrated into the cell’s genome or when introduced into the cell as an extrachromosomal element, is capable of expressing a fluorescent biosensor to measure changes in the NAD + / NADH ratio within cells and contains the nucleic acid according to claim 1 under the control of regulatory elements necessary for expression nucleic acid in the host cell. 3. A cell producing a biosensor encoded by a nucleic acid according to claim 1, containing an expression cassette according to claim 2 in the form of an extrachromosomal element or an element integrated into the genome of this cell. An isolated fluorescence biosensor for measuring changes in the NAD + / NADH ratio inside cells encoded by the nucleic acid according to claim 1, wherein said biosensor responds by increasing the signal when the NAD + / NADH ratio shifts to a decrease in the NADH concentration. |
| priorityDate | 2012-03-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
| Predicate | Subject |
|---|---|
| isDiscussedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/compound/CID5892 http://rdf.ncbi.nlm.nih.gov/pubchem/substance/SID419547023 |
Total number of triples: 10.