http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2011144738-A
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K35-50 |
| filingDate | 2011-11-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2013-05-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | RU-2011144738-A |
| titleOfInvention | METHOD FOR PRODUCING AND CLEANING PLACENTAL ALKALINE PHOSPHATASE |
| abstract | The method of obtaining and purification of placental alkaline phosphatase, which consists in extracting the enzyme from raw materials, heat treatment of the extract, fractionation of proteins with ammonium sulfate and subsequent purification of the enzyme by preparative electrophoresis, characterized in that after extraction of the enzyme from the placenta with 3M potassium chloride and heating of the primary extract for 10 min at 65 ° C, repeated heat treatment of the extract is carried out in the presence of cadmium sulfate of 0.14% concentration for 1-3 minutes at 65 ° C, followed by cooling to foreheads under a stream of water, ammonium sulfate is added to the extract to 30% saturation, the precipitate is separated by centrifugation, the supernatant, bypassing the dialysis stage, is subjected to hydrophobic chromatography on a phenyl-sepharose column, the enzyme is eluted from the column with 0.02 M Tris-glycine electrode pH buffer 8.6, and the enzyme-containing eluate is subjected to preparative electrophoresis in 7% polyacrylamide gel. |
| priorityDate | 2011-11-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 18.