http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2011140251-A
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2317-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-952 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-33 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y304-24069 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2317-92 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-96425 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-47 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2317-56 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-18 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-53 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-5035 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-573 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-5014 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y304-24069 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-37 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-52 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-50 |
| filingDate | 2010-03-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2013-04-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | RU-2011140251-A |
| titleOfInvention | IMMUNOLOGICAL TESTS FOR THE ACTIVITY OF ENDOPEPTIDASIS WITH A CHANGED FOCUS |
| abstract | 1. A method for detecting activity of endopeptidases with a changed focus, comprising the steps of: a. treating a cell from a stable cell line with a sample containing an altered targeting endopeptidase, said cell from a stable cell line being sensitive to the activity of said endopeptidase with an altered focus at an endopeptidase concentration with an altered focus of about 500 pM or lower; b. isolating from the treated cell the SNAP-25 component containing the SNAP-25 cleavage product, the carboxyl end of which corresponds to the glutamine of the cut bond at the BoNT / A toxin cleavage site; c. contacting the SNAP-25 component with an anti-SNAP-25 antibody immobilized on a solid phase support, while the anti-SNAP-25 antibody binds to an epitope whose carboxyl end corresponds to the glutamine of the cut bond in the BoNT / A toxin cleavage site from the SNAP cleavage product -25, anti-SNAP-25 antibody is characterized by an association rate constant for an epitope of SNAP-25 that does not contain the carboxy-terminal glutamine of the cleaved bond at the BoNT / A toxin cleavage site, from the SNAP-25 cleavage product of less than 1 × 10 Ms; and the anti-SNAP-25 antibody is characterized by an equilibrium dissociation constant for the epitope of less than 0.450 nM; g. detecting the presence of an antibody-antigen complex comprising an anti-SNAP-25 antibody and an SNAP-25 cleavage product, the carboxyl end of which corresponds to the glutamine of the cleaved bond at the BoNT / A toxin cleavage site; wherein detection with the indicated antibody-antigen complex is an indicator of endopeptidase activity with a changed focus |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2683207-C2 |
| priorityDate | 2009-03-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 50.