http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2011116878-A
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y02A50-30 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-20 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-04 |
| filingDate | 2011-04-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2012-11-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | RU-2011116878-A |
| titleOfInvention | METHOD FOR DIFFERENTIATION OF VIBRIO CHOLERAE O139 STRAINS SERO GROUPS ON ALKYL SULPHATIC ACTIVITY |
| abstract | The method of differentiation of strains of Vibrio cholerae O139 serogroup by alkyl sulfatase activity, including the use of sodium dodecyl sulfate as a substrate in a nutrient medium, characterized in that they create a synthetic medium for the preparation of which per 100 ml is necessary: 0.5 g of sodium chloride, 2 g of agar, 0.002 g of bromothymol blue indicator, then the components are dissolved in 0.01 M Tris HCl buffer, having a pH of 7.8 and subjected to boiling for 30 minutes, obtaining a molten mass into which 0.1 g of sodium dodecyl sulfate substrate is introduced, then about The agar cultures under study are inoculated onto the obtained synthetic medium, followed by incubation at 37 ° C for 24-48 hours, after which they are differentiated by the alkyl sulfatase activity of the serogroup O139 cholera vibrios, which hydrolyze the sodium dodecyl sulfate substrate; therefore, agar sectors around the seed are clearly visible turbid - white zones 2-10 mm in size, which confirms the toxigenicity of the strain and its epidemiological significance, and if there are no such zones, they conclude that the strain is non-toxic and its epidemic insignificance. |
| priorityDate | 2011-04-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 22.