http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2010146963-A

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filingDate 2009-04-16-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationDate 2012-05-27-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber RU-2010146963-A
titleOfInvention PROTEIN HYDROLYSATE CLEANING AND RESULTED PRODUCTS
abstract 1. A method of purifying an enzymatically cleaved protein hydrolyzate obtained from the production of heparin (peptone), comprising the step of passing peptone through a nanofilter at a temperature in the range of from about room temperature to about 130 ° F and a pressure in the range of atmospheric to about 360 psi to obtain as a result of peptone concentrate. ! 2. The method according to claim 1, comprising the subsequent stage of evaporation of the peptone concentrate to a predetermined percentage of moisture. ! 3. The method according to claim 1, including the stage! a) acidifying the peptone to a pH in the range of from about 4 to about 7 to obtain a precipitate layer including fatty and / or flocculated components and an aqueous layer! b) separation of the sediment layer from the water layer,! c) passing the aqueous layer through the nanofilter at a temperature in the range of from about room temperature to about 130 ° F. and a pressure in the range of atmospheric pressure to about 360 psi, resulting in peptone concentrate (1). ! 4. The method according to any one of claims 1 to 3, where the pore size of the nanofilter has a nominal molecular weight cut-off in the range of 150-300 Da. ! 5. The method according to any one of claims 1 to 3, where in the next step the permeate obtained in the nanofiltration step is again passed through the nanofilter to obtain peptone concentrate (2). ! 6. The method according to claim 5, where the nanofilter has a pore size that allows a nominal cut-off by molecular weight <150 Da. ! 7. The method according to any one of claims 1 to 3, wherein softened water is added to the peptone concentrate, for example the peptone concentrate (1), and the resulting composition is again passed through a nanofilter to obtain a peptone concentrate (4). ! 8. The method according to claim 5, where the peptone concentrate
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