http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2010142477-A
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-48 |
| filingDate | 2010-10-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2012-04-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | RU-2010142477-A |
| titleOfInvention | METHOD FOR FORECASTING THE INDIVIDUAL LEVEL OF DEVELOPMENT OF PATHOLOGIES ASSOCIATED WITH AGING ON THE BASIS OF MEASURING THE LENGTH OF PERIPHERAL BLOOD CELLS |
| abstract | A method for predicting the individual level of development of pathologies associated with aging, based on measuring the length of the telomeres of peripheral blood cells, including collecting peripheral blood from a vein and placing a sample of this blood in an anticoagulant tube, obtaining nucleated cells, using control cells for comparison, resuspension in a hybridization solution, containing a peptide-nucleic probe, a mixture of the obtained peripheral blood cells with control cells, after washing the mixture and staining in a solution containing propidium iodide analysis for measuring the telomere length of cells, characterized in that after sampling the peripheral blood from a vein by means of a vacuum system and placing it in a test tube with an anticoagulant K2-ethylenediaminetetraacetic acid, 0.5-1 ml of cell leukoprecipitate is obtained by settling blood for 30-50 minutes at the vertical position of the tube, the number of peripheral blood leukemia cells and control cells taken as a cell culture of the T-lymphoblastoid line are aligned leukemia 1301, cultivated in a special medium for culturing suspension cells of the Roswell Pack Metorial Institute medium with 10% bovine serum, glutamine and penicillin with streptomycin, by washing them in a solution of phosphate-buffered saline with the addition of 0.1% bovine serum albumin and after mixing, their number and viability are determined on a Vi-CELL ™ cell analyzer, isolating the required number in the range of 0.5-3 million, then half of the cells of the peripheral blood leukemia and the cell line 1301 are placed in |
| priorityDate | 2010-10-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 26.