http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2010130462-A

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filingDate 2008-12-19-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationDate 2012-01-27-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber RU-2010130462-A
titleOfInvention PROTEIN-FREE HAMETA AND EMBRYO PROCESSING AND CULTURAL MEDIA PRODUCTS
abstract 1. Essentially protein-free cell culture medium for human reproductive cells, suitable for use in the processing of in vitro human reproductive cells, which are used in in vitro fertilization and other assisted reproductive technologies, containing mineral salts, amino acids, antioxidants, vitamins, nutritious substances, antibiotics, D-mannitol and methyl cellulose, which has a molecular weight of 14,000 daltons. ! 2. Essentially protein-free medium according to claim 1, wherein said methyl cellulose is characterized in that a 2% solution has a viscosity of 15 centipoise at 25 ° C. ! 3. Essentially, the protein-free medium according to claim 1, wherein said methyl cellulose has the formula I:! ! in which each R is independently H or CH3, and n is an integer having a value of from about 34 to about 43, and in which the methoxy substitution is from 27.5 to 31.5 wt.%. ! 4. Essentially protein-free medium according to claim 4, in which the average number of CH3 substituents attached to each sugar unit of the compound of formula I is from 1.5 to 1.9. ! 5. Essentially protein-free medium according to claims 1, 2, 3 or 4, wherein said methyl cellulose is present in solution in a concentration of from 0.01 g / l (0.71 μM) to 0.5 g / l (0.036 mm ) ! 6. Essentially protein-free medium according to claims 1, 2, 3 or 4, in which the amino acids are L-arginine, L-cystine, L-histidine, L-isoleucine, L-leucine, L-lysine, L-methionine , L-phenylalanine, L-threonine, L-tryptophan, L-tyrosine, L-valine, L-alanine, L-taurine, L-glutamic acid, L-glutamine or glycine, or any combination thereof. ! 7. Essentially protein-free medium according to claims 1, 2, 3 or 4, in which the mineral salts included in the composition
priorityDate 2007-12-21-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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