http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2010130453-A
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8213 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-11 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-87 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8206 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-87 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-82 |
| filingDate | 2007-12-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2012-01-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | RU-2010130453-A |
| titleOfInvention | IMPROVED METHOD OF MUTAGENESIS USING POLYETHYLENE Glycol-MEDIATED ADMINISTRATION OF MUTAGENIC NUCLEINE BASES IN PLANT PROTLASTES |
| abstract | 1. A method for directionally changing a duplex acceptor DNA sequence in a protoplast of a plant cell, comprising combining a duplex acceptor DNA sequence with a donor mutagenic nucleic base, where the duplex acceptor DNA sequence contains a first DNA sequence and a second DNA sequence that is complementary to the first DNA sequences, and where the donor mutagenic nucleic base includes at least one erroneous pair in relation to duplex acceptance a DNA sequence to be changed, preferably with respect to the first DNA sequence, where the method further comprises the step of introducing the mutagenic nucleic base into the cell protoplasts using polyethylene glycol (PEG) -mediated transformation. ! 2. The method according to claim 1, where the mutagenic nucleic acid base is a mutagenic single-stranded nucleic acid base. ! 3. The method according to claim 1, where the mutagenic nucleic base includes LNA substitutions, which are at least one nucleotide deleted from an erroneous target pair, and, optionally, at least 3, 4 or 5 nucleotides removed from 5 '- and 3'-ends of the mutagenic nucleic base. ! 4. The method according to claim 1, where the mutagenic nucleic base includes propyne substitutions. ! 5. The method according to claim 1, where the acceptor DNA is taken from genomic DNA, linear DNA, artificial mammalian chromosomes, bacterial artificial chromosomes, yeast artificial chromosomes, plant artificial chromosomes, nuclear chromosome DNA, organelles chromosome DNA, episomal DNA. ! 6. |
| priorityDate | 2007-12-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
| Predicate | Subject |
|---|---|
| isDiscussedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/substance/SID411502039 http://rdf.ncbi.nlm.nih.gov/pubchem/compound/CID6335 |
Total number of triples: 16.