http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2010119558-A
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-33 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2510-02 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-47 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-303 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 |
| filingDate | 2008-10-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2011-11-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | RU-2010119558-A |
| titleOfInvention | METHOD FOR PRODUCING A CELL THAT IS ABLE TO PRODUCE HETEPROTEINS WITH A HIGH OUTPUT |
| abstract | 1. A method of producing a cell capable of producing the desired polypeptide in high yield, in which a cell with strong expression of the taurine transporter, into which DNA encoding the desired polypeptide has been introduced, is cultured in the presence of a high concentration of methotrexate, and a cell capable of producing desired polypeptide in high yield. ! 2. The method according to claim 1, in which a DNA encoding a dihydrofolate reductase is also introduced into a cell with strong expression of a taurine transporter into which DNA encoding the desired polypeptide has been introduced. ! 3. The method according to claim 2, in which a cell with strong expression of a taurine transporter into which DNA encoding the desired polypeptide has been introduced is a cell cotransformed with one molecule containing DNA encoding the desired polypeptide and DNA encoding dihydrofolate reductase. ! 4. The method according to claim 3, in which the molecule containing the DNA encoding the desired polypeptide and the DNA encoding the digitrofolate reductase is a vector. ! 5. The method according to any one of claims 1 to 4, in which the cell with strong expression of the taurine transporter also strongly expresses cysteinesulfinic acid decarboxylase. ! 6. A cell obtained by the method according to any one of claims 1 to 5. ! 7. The method of obtaining the desired polypeptide, in which the cell is cultured according to claim 6. ! 8. The method according to claim 7, in which the desired polypeptide is an antibody. ! 9. A method of obtaining a pharmaceutical product containing a polypeptide obtained by the method according to claim 7 or 8.! 10. Method for quantitatively increasing the production of a polypeptide by a cell with strong expression of the taurine transporter into which DNA was introduced |
| priorityDate | 2007-10-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 27.