http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2010115908-A
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H21-00 |
| filingDate | 2010-04-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2011-10-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | RU-2010115908-A |
| titleOfInvention | METHOD FOR SIMULTANEOUS AUTOMATED ISOLATION AND PURIFICATION OF NUCLEIC ACIDS FROM SEVERAL BIOLOGICAL SAMPLES |
| abstract | 1. A method for the simultaneous automated isolation and purification of nucleic acids from several biological samples, characterized in that the isolation and purification of nucleic acids is carried out in disposable modules containing all the reagents necessary for the destruction of cells, microorganisms and viral particles, purification and elution of nucleic acids, and located on a rotating platform, and the movement of liquids inside the modules is carried out either under the action of forces arising from the rotation of the platform, or when creating The pressure difference in the respective tanks modules, and the isolation and purification of nucleic acids includes the following steps: a) the first stage of the lysis of cells, microorganisms and viral particles using the first lysis buffer containing lysozyme or its analogues; ! b) the second stage of the lysis of cells, microorganisms and viral particles using a second lysis buffer containing a chaotropic agent, proteolytic enzyme, detergent; ! c) adding alcohol to the obtained lysate of the biological sample to create optimal conditions for the binding of nucleic acids to a solid-phase sorbent; ! d) nucleic acid binding on a solid phase sorbent; ! d) washing the nucleic acids associated with the solid-phase sorbent from impurities by sequentially passing a wash buffer containing a chaotropic agent and a water-alcohol mixture; ! f) elution of nucleic acids from a solid-phase sorbent by passing an eluting solution. ! 2. The method according to claim 1, characterized in that the isolation and purification of nucleic acids is carried out simultaneously from two or more biolog |
| priorityDate | 2010-04-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 50.