http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2010102146-A
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K17-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K38-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/B01J20-281 |
| filingDate | 2010-01-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2011-08-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | RU-2010102146-A |
| titleOfInvention | METHOD FOR IMMUNOAFFINE SORBENT SYNTHESIS FOR ISOLATION OF HUMAN ALPHA-FETOPROTEIN |
| abstract | 1. The method of synthesis of the immunoaffinity sorbent for the isolation of human alpha-fetoprotein, which consists in the activation of the carbohydrate matrix (gel) by adding a 0.07 M sodium periodate solution and after 45 minutes stirring at room temperature, add 2 M ethylene glycol solution and mix again at the same temperature for 30 minutes, followed by washing with purified water and potassium bisphosphonate buffer solution in series. ! 2. The method according to claim 1, characterized in that the addition of the AFP protein with the activated matrix with constant stirring is performed for 48 hours at 4-8 ° C in the dark. ! 3. The method according to claim 1 or 2, characterized in that the washing of the matrix with sewn anti-AFP antibodies is carried out with ice-cold PBS 0.15 M pH 7.25; blocking unreacted groups with sodium borohydride while slowly stirring for 1 h at 4 ° C in a dark dish; PBS is washed with 0.15 M pH 7.25, then washed with chilled KBB 0.1 M pH 8 per 0.5 M sodium chloride and chilled acetate buffer alternately three times, then washed with 0.15 M sodium chloride 400 ml and a chaotropic reagent (magnesium chloride 2.8 M pH 2.3 with 0.5% Triton X-100); it is then washed with 0.15 M sodium chloride, the immuno-affinity component thus obtained is preserved in PBS 015 M pH 7.25 with azide and a semisynthetic penicillin antibiotic of at least 2 L. |
| priorityDate | 2010-01-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 48.