http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2010100910-A
Outgoing Links
Predicate | Object |
---|---|
classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01K2207-15 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01K2217-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01K2267-01 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01K2227-101 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2830-008 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8509 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8771 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01K67-0278 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-62 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-02 |
filingDate | 2008-06-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2011-07-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | RU-2010100910-A |
titleOfInvention | METHOD FOR PRODUCING INSULIN (OPTIONS) |
abstract | 1. A method of producing insulin, comprising creating a non-human transgenic mammal that forms a modified insulin precursor in milk, obtaining milk from said mammal, purifying the modified insulin precursor from milk, resulting in a pure modified insulin precursor, converting said insulin precursor to insulin and purification of insulin. ! 2. The method of claim 1, wherein the non-human transgenic mammal is created by cloning a nucleic acid sequence that encodes a modified insulin precursor into a plasmid whereby the sequence is operably linked to a promoter that will direct expression of the sequence in the cells of the mammary gland resulting in an expression plasmids transfect somatic cells with an expressing plasmid, incorporating the plasmid into the somatic genome their cells, resulting in transgenic somatic cells, enucleate a mature egg cell to obtain an egg cell with the nucleus removed, carry out the fusion of one of the somatic cells with the indicated egg cell, leading to the formation of a unicellular embryo, implant the embryo into the uterus of the recipient mammal and monitor pregnancy before the transgenic a mammal. ! 3. The method according to claim 2, in which the promoter is a β-casein promoter, and the insulin and the modified insulin precursor are mammalian insulin and the modified mammalian insulin precursor, respectively |
priorityDate | 2007-06-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 121.