http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2009149040-A
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K39-00 |
| filingDate | 2009-12-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2011-07-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | RU-2009149040-A |
| titleOfInvention | METHOD FOR OBTAINING COMBINED BIVALENT, CULTURAL, INACTIVATED, CONCENTRATED, CLEANED VACCINES FOR PREVENTION OF HEMORRHAGIC FEVER WITH RENAL SYNDROME |
| abstract | 1. A method for producing a combined bivalent, cultural, inactivated, concentrated, purified vaccine for the prevention of hemorrhagic fever with renal syndrome by reproducing the PUMUMALA and WELL virus strains in a monolayer transplantable culture of green monkey kidney cells, VERO line, including microfiltration of a virus-containing to remove cell detritus, inactivation of the virus with formalin, concentration by ultrafiltration and purification of inactivated concentrate from ball ACTH proteins chromatography sorption on aluminum hydroxide. ! 2. The method according to claim 1, characterized in that for infection of the cell culture using strains of viruses "PUMALA" and "GOOD", adapted for propagation in cell culture VERO. ! 3. The method according to claim 1, characterized in that the reproduction of viruses on a transplantable cell culture of VERO is achieved without the addition of serum of the fetal cattle. ! 4. The method according to claim 1, characterized in that to obtain the viral mass, a five-fold collection of virus-containing liquid is carried out from one monolayer of cells. ! 5. The method according to claim 1, characterized in that microfiltration is carried out on filters with a pore size of from 0.6 to 0.22 μm. ! 6. The method according to claim 1, characterized in that specific safety is achieved by inactivation of a live virus with a 0.025% formalin solution at a temperature of 6 ± 2 ° C. ! 7. The method according to claim 1, characterized in that the inactivated antigen-containing culture fluid is subjected to concentration by ultrafiltration in a tangential flow using a concentration system with a cutoff limit of 100 kDa and a filtration area of 0.1 to 0.5 m2. ! 8. Spos |
| priorityDate | 2009-12-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 43.