http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2009142111-A
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-20 |
| filingDate | 2009-11-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2011-05-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | RU-2009142111-A |
| titleOfInvention | METHOD FOR PRODUCING MICROBIAL ELASTASIS |
| abstract | 1. A method of producing microbial elastase by growing strains of microorganisms in a nutrient medium, characterized in that in it the seed of the strain of actinomycete Streptomyces lavendulae VKP-S-910 is placed on a nutrient medium of the following composition, g / l:! corn flour 20-22 flour animal feed 10-12 MgSO47H2O 0.04-0.06 water pH 6.5-8.5 rest,! when the ratio of nutrient medium and seed material of strain 10: 1, and the resulting mixture in portions of 50 ml is placed in Erlenmeyer flasks with a volume of 250 ml on a rocking chair and the strain is grown in a flask rotation mode of 200 rpm at a temperature of 25-30 ° C for 72 hours after which the resulting native solution is separated from the Lyceum biomass by centrifugation at 5000 ± 50 rpm and precipitated at a temperature of 0-5 ° C with an 80% solution of ammonium sulfate during subsequent dialysis against distilled water. ! 2. The method according to claim 1, characterized in that to determine the level of activity of the elastolytic activity of the obtained enzyme, 1 ml of the test solution is mixed with 50 mm of glycirin in NaCL-NaOH-buffer pH 8.5 containing 20 mg of elastin stained with orcin, and after incubation of the mixture by shaking for 20 min at a temperature of 37 °, to stop the reaction add 2 ml of 0.7 M phosphorus buffer pH 6.0, after which insoluble elastin is removed from the mixture in a centrifuge and the optical density of the solution is measured on a spectrophotometer at 590 nm. ! 3. The method according to claim 1, characterized in that in it one unit of elastolytic activity is defined as the amount of enzyme that breaks down 1 mg of elastin substrate in 1 h. |
| priorityDate | 2009-11-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 32.