http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2009127816-A
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K38-16 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-18 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-775 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-00 |
| filingDate | 2007-09-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2011-01-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | RU-2009127816-A |
| titleOfInvention | METHOD FOR CLEANING APOLIPOPROTEIN A-1 |
| abstract | 1. A method for purifying apolipoprotein A-1, including the stages:! a) mixing of plasma fraction IV with a solution of 1-8 M urea, forming a preparatory solution of fraction IV; ! b) loading the preparatory solution obtained in step a) into the first column for anionic chromatography, and subsequent elution with buffer I containing 1-8 M urea, having a conductivity of 1-4 ms / cm, to obtain eluent I, which is mainly contains apoA-1 protein; ! c) loading eluent I onto a second anion chromatography column, first elution with buffer II having a conductivity of 1-15 msec / cm and then elution with buffer III having a conductivity of 30-100 msec / cm to obtain pure apoA-1 protein. ! 2. A process according to claim 1, wherein the plasma fraction IV is obtained by a low temperature ethanol fractionation process. ! 3. The method of claim 1, wherein the buffer II comprises 0-1 M urea. ! 4. The method of claim 1, wherein the buffer III comprises 0-1 M urea. ! 5. The method according to claim 1, wherein the concentration of urea in steps a) and b) is 3-8 M.! 6. The method according to claim 1, wherein the concentration of urea in steps a) and b) is 5-7 M.! 7. The method of claim 1, wherein the conductivity of buffer I is 2-4 msec / cm, the conductivity of buffer II is 7-15 msec / cm, and the conductivity of buffer III is 70-95 msec / cm. ! 8. A method according to claim 1, wherein the conductivity of buffer I is 2.5-3.6 msec / cm, the conductivity of buffer II is 9-12 msec / cm, the conductivity of buffer III is 80-90 msec / cm. ! 9. The method of claim 1, wherein each anion exchange chromatography column is one of 1) a high performance ion exchange chromatography column and 2) a low performance ion exchange chromatography column. ! 10. The method according to claim 1, wherein each column for an |
| priorityDate | 2006-12-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 93.