http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2008151507-A
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2795-00034 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2320-32 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K2039-5258 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2795-18122 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2310-17 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2795-18123 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P37-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-117 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07H21-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N7-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-86 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N7-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-117 |
| filingDate | 2007-06-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2010-07-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | RU-2008151507-A |
| titleOfInvention | METHODS FOR PACKING OLIGONUCLEOTIDES IN VIRUS-LIKE PARTICLES OF RNA-CONTAINING BACTERIOPHAGES |
| abstract | 1. A method of obtaining a nucleotide composition containing an oligonucleotide, which includes the following stages:! (a) providing an oligonucleotide in solution I, wherein said oligonucleotide contains at least one poly-G region, wherein said solution I has an alkaline pH, preferably said pH is 8-13, more preferably said pH is 12,! (b) the disaggregation of said oligonucleotide, said disaggregation comprising the steps of:! (i) adjusting the temperature of solution I to temperature I, said temperature I being 4-70 ° C, preferably 45-70 ° C, more preferably about 50 ° C, and most preferably 50 ° C,! (ii) incubating said oligonucleotide in said solution I at said temperature I, said incubation being carried out until said oligonucleotide has a relative peak onset time of about 110%, and! (iii) controlling the temperature of said solution I to temperature II, wherein said temperature II is 0-70 ° C, preferably said temperature II is lower than temperature I, and also preferably temperature II is 0-25 ° C, most preferably 0-2 ° C,! (c) adjusting the pH of said solution I to a value of 5-8, preferably said regulation of said pH of said solution I is carried out until said pH is 6-7, and! (g) aggregating said oligonucleotide, said aggregation comprising the steps of:! (i) providing said oligonucleotide in solution II, wherein the pH of said solution II is 5-8 and said solution |
| priorityDate | 2006-06-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 162.