http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2008133987-A
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-15 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2502-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-115 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P25-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-24 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0664 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P19-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0607 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P21-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P35-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P25-28 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P43-00 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-071 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-077 |
| filingDate | 2007-02-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2010-02-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | RU-2008133987-A |
| titleOfInvention | METHODS FOR COLLECTING AND SELECTING A POPULATION OF EMBRYON-LIKE STEM CELLS FROM A PERIODONTAL FOLICULAR TISSUE OF AN ADULT PERSON |
| abstract | 1. A method for isolating, building and storing a population of stem cells that originate from a human dental follicle and are called FENC (stem cells derived from the follicles of the germinal neural crest stem cells), comprising:! a) collecting the follicular sac in sterile conditions, splitting, growing and building up the primary culture; ! b) optional amplification; ! c) sorting by FAC sorter. ! 2. The method according to claim 1, where the cleavage is carried out using proteolytic enzymes and antibodies. ! 3. The method according to claim 2, where the enzymatic digestion is carried out by means of an aqueous solution containing: 3 mg / ml type I collagenase, 4 mg / ml dispase, 100 units / ml penicillin, 100 μg / ml streptomycin and 500 μg / ml clarithromycin in PBS. ! 4. The method according to claim 2, where the enzymatic cleavage is carried out for 1 hour at 37 ° C with shaking. ! 5. The method according to claim 1, where the growth and extension of the primary culture is carried out using a culture medium specialized for stem cells. ! 6. The method according to claim 5, where the culture medium consists of Mega Cell, supplemented with 10% fetal bovine serum (FBS), 100 μm 2-phospho-ascorbic acid, 2 mm L-glutamine, 100 units / ml penicillin, 100 μg / ml streptomycin. ! 7. The method according to claim 5, where the incubation occurs at 37 ° C in an atmosphere of 5% CO2 for 15-20 days, with a change of medium every 3 days. ! 8. The method according to any one of claims 1 to 7, wherein the FAC sorting is performed using antibodies to SSEA-4, TRA 1-60, TRA 1-81, CD133, CD90, flk-1 as stem cell markers. ! 9. The use of FENC obtained by the method according to any one of claims 1 to 8, for the production of osteogenic precursors, osteoblasts, myoblasts, chondrocytes, adipocytes, neurons, glial cells. ! 10. Applied |
| priorityDate | 2006-02-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 34.