http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2008124890-A
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y02A50-30 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07G3-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07H15-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K35-30 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P25-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01D15-08 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K31-7032 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K31-715 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07H17-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K31-351 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K31-7032 |
| filingDate | 2008-06-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2009-12-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | RU-2008124890-A |
| titleOfInvention | METHOD FOR PRODUCING PURE GM1 MONOSIALOGANGLIOSIDE FOR USE IN MEDICINE |
| abstract | 1. The method of isolation and purification of monosialoganglioside GM1, comprising the steps of:! (a) isolating GM1 from a lipid mixture containing GM1 monosialoganglioside as the main ganglioside component using ion exchange column chromatography using an eluent containing potassium or cesium ions,! (b) extracting the solute from the eluted solution,! (c) diafiltration of the aqueous solution of the extracted solute of step (b),! (g) adding sodium salt and diafiltration of the resulting aqueous solution,! (e) extracting GM1 in the form of its sodium salt. ! 2. The method according to claim 1, in which the eluent contains cesium or potassium acetate. ! 3. The method according to claim 2, in which the eluent is a methanol solution of cesium or potassium acetate. ! 4. The method according to claim 1, in which the ion exchange column contains a resin having a Quaternary amino group. ! 5. The method according to claim 1, in which the ion exchange column is first balanced with methanol. ! 6. The method according to claim 1, in which NaCl is added in stage (g). ! 7. The method according to claim 1, in which diafiltration is carried out using a membrane having a pore size of 10000-100000 daltons. ! 8. The method according to claim 1, in which the extraction of GM1 in stage (e) includes drying the permeate solution by spray drying or vacuum drying to form a powder. ! 9. The method according to claim 1, comprising the stage of preliminary purification of GM1 before separation by ion exchange chromatography, and the specified stage of purification includes:! (a) diafiltration of an aqueous solution of a lipid mixture containing GM1 monosialoganglioside as the main ganglioside component, through a membrane having a pore size of 10000-100000 daltons,! (b) concentrated permeate and solute recovery� |
| priorityDate | 2007-06-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 198.