| abstract |
1. A method for producing human stem cells, including! a) parthenogenetic activation of the human oocyte, where activation includes i) contacting the oocyte with the ionophore at high oxygen pressure (O2) and ii) contacting the oocyte with an inhibitor of serine-threonine kinase at a low pressure of O2; ! b) culturing the activated oocyte from step a) at low O2 pressure until a blastocyst is formed; ! c) transfer of the blastocyst to the layer of feeder cells and cultivation of the transferred blastocyst at high pressure O2; ! d) mechanical separation of the cell mass of the inner region (ICM) from the trophectoderm of the blastocyst from stage c); and! e) culturing ICM cells from step d) on a layer of feeder cells,! where ICM culture step (e) is carried out at a high pressure of O2. ! 2. The method according to claim 1, in which the low pressure of O2 is maintained by incubation in a medium of a gas mixture containing O2 in a concentration of approximately 2% O2 to 5% O2. ! 3. The method according to claim 2, wherein the gas mixture medium further comprises about 5% CO2 and about 90% N2 to 93% N2. ! 4. The method according to claim 1, in which a high pressure of O2 is maintained by incubation in a medium of a gas mixture containing about 5% CO2 and about 20% O2. ! 5. The method according to claim 1, in which the ionophore is selected from the group consisting of ionomycin and A23187. ! 6. The method according to claim 1, wherein the serine-threonine kinase inhibitor is selected from the group consisting of staurosporine, 2-aminopurine, sphingosine and 6-dimethylaminopurine (DMAP). ! 7. The method according to claim 1, in which the medium contains serum from the umbilical cord of a person. ! 8. The method according to claim 1, in which the layer of nourishing cells contains human fibroblasts. ! 9. The method of claim 8, in which |