http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2008107953-A
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y02P20-52 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-00 |
| filingDate | 2008-03-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2009-09-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | RU-2008107953-A |
| titleOfInvention | METHOD FOR PRODUCING A HETEROGENEOUS BIO-CATALYST CATALYST, A BIO-CATALYST BASED ON HYDROLASE OF ALPHA-AMINO ACIDS ETHERS AND A METHOD OF SYNTHESIS OF AMINOCEPHALOSPORINES AND AMINOPENICYLINOISOLYTOLITOMIS BITOMIS |
| abstract | 1. A method of producing a heterogeneous biocatalyst based on an intracellular hydrolase of alpha-amino acid esters from bacteria of the genus Xanthomonas, including the destruction of cell biomass, extraction of the enzyme from it into the aqueous phase and its subsequent immobilization, characterized in that the biomass of Xanthomonas rubrilineans bacteria cells is used; cell destruction is carried out in stages, first violating their integrity by cryogenic exposure, and then, treating the aqueous suspension of thawed cells with butyl acetate; the enzyme is extracted into the aqueous phase by heating the treated cell suspension, first under conditions of a spontaneously established pH gradient, and then at a constant pH maintained by adding a titrant solution until the extraction procedure is stopped, using the degree of solution flow rate change as a criterion for choosing the stopping moment titrant; the cell-free extract obtained after separation of the destroyed cellular biomass is used without any purification for the procedure of immobilization of the enzyme by its deposition in the form of enzyme aggregates and their subsequent cross-linking. ! 2. The method according to claim 1, characterized in that the cryogenic effect on the cells is carried out by freezing the biomass to a temperature of -25 ° C or lower, preferably from -25 to -30 ° C. ! 3. The method according to claim 1, characterized in that for the treatment of cells with butyl acetate, an aqueous suspension with a cell biomass concentration of 20 ÷ 100 mg dry / ml is used. ! 4. The method according to claim 1, characterized in that butyl acetate is used in a concentration of 2 ÷ 6 vol.%. ! 5. The method according to claim 1, characterized in that the extraction of the enzyme is carried out at a temperature of 25 ÷ 35 ° C. ! |
| priorityDate | 2008-03-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 15.