http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2008103319-A
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2510-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-90 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2800-107 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2820-60 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2820-007 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P21-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P21-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-8125 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-6427 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-85 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-79 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-67 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-6437 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-644 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y304-21021 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y304-21022 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-755 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-535 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-54 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-505 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y304-21001 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-47 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-07 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-79 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-67 |
| filingDate | 2006-06-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2009-08-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | RU-2008103319-A |
| titleOfInvention | SCAM-FREE STABLE TRANSFECTION AND PRODUCTION OF RECOMBINANT HUMAN PROTEINS IN HUMAN CELL LINES |
| abstract | 1. A method for producing an immortalized human cell line stably transfected with a nucleic acid sequence containing a gene encoding a target human protein, or a derivative thereof, or a mutant thereof, a promoter and a polyadenylation signal (polyA) of bovine growth hormone, wherein said promoter and polyA signal are associated with The 5'- and 3'-end of the gene encoding the specified target human protein, respectively, where the method includes transfection of the immortalized line of human host cells in serum-free conditions with a transfection vector and comprising said nucleic acid sequence and origin of replication. ! 2. The method according to claim 1, in which the target human protein is a plasma protein of a person selected from coagulation factors, such as factor IX, factor VIII (wild-type and with a deletion of the B domain), factor VII / VIIa and factor von Willebrand (vWF), growth factors such as erythropoietin, colony stimulating factors (CSF) such as granulocyte-stimulating factor (G-CSF), macrophage CSF (M-CSF) and granulocyte macrophage CSF (GM-CSF), cytokines such as interleukins, protease inhibitors such as alpha-1 antitrypsin (A1AT) and chymotrypsin, transport proteins such as hormones proteins with inhibitory or regulatory action and their derivatives and mutants, preferably the human protein is selected from factor IX, factor VIII, including wild-type factor VIII and factor VIII with a deletion of the B domain, factor VII / VIIa, G-CSF, vWF and A1AT, most preferably a human protein, is coagulation factor IX encoded by bp 939-2324 SEQ ID NO: 1, human A1AT encoded by bp 973-2259 SEQ ID NO: 2, wild-type factor VIII shown in SEQ ID NO: 9, human factor VIII with deletion B |
| priorityDate | 2005-06-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 381.