http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2007149047-A
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-25 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 2006-02-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2009-07-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | RU-2007149047-A |
| titleOfInvention | METHOD FOR DETECTION OF MICROORGANISM AND KIT FOR DETECTION OF MICROORGANISM |
| abstract | 1. A method for detecting living cells of a microorganism in a test sample, which includes the following stages:! a) the stage of processing the test sample with topoisomerase poison and / or DNA gyrase poison,! b) the stage of DNA extraction from the test sample and the amplification of the target region of the extracted DNA by PCR and! c) a step for analyzing the amplification product. ! 2. The method according to claim 1, where the amplification product is analyzed using a standard curve that reflects the dependence of the amount of amplification product on the amount of microorganism, which is obtained using standard samples of the microorganism. ! 3. The method according to claim 2, where the PCR is carried out in real time simultaneously with the analysis of the amplification product. ! 4. The method according to any one of claims 1 to 3, where the test sample is milk, a dairy product, a food product obtained using milk or a dairy product as a raw material, a blood sample, a urine sample, a cerebrospinal fluid sample, a synovial fluid sample, and pleural fluid sample. ! 5. The method according to any one of claims 1 to 3, where the microorganism is a bacterium. ! 6. The method according to claim 5, where the target region is a 23S rRNA gene. ! 7. The method according to claim 6, where the PCR is carried out using a set of primers containing primers SEQ ID NO: 1 and 2, or a set of primers containing primers SEQ ID NO: 3 and 4.! 8. The method according to any one of claims 1 to 3, where the microorganism is a pathogenic bacterium. ! 9. The method of claim 8, where the target region is preferably a pathogenic gene. ! 10. The method according to claim 9, where the PCR is preferably carried out using a set of primers containing primers SEQ ID NO: 7 and 8.! 11. The method according to any |
| priorityDate | 2006-02-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 44.