http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2007146777-A
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0606 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-0735 |
| filingDate | 2006-05-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2009-06-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | RU-2007146777-A |
| titleOfInvention | OBTAINING A LINE OF HUMAN EMBRYONIC STEM CELLS USING MAMMAL CELLS |
| abstract | 1. A purified preparation of human pluripotent embryonic stem cells, where the cells have ! (i) the ability to differentiate into endoderm, mesoderm and ectoderm tissue derivatives, ! (ii) normal karyotype, ! (iii) the ability to propagate in in vitro culture for at least about 25 passages, and ! (iv) one or more human leukocyte antigen (HLA) alleles from Table 4. ! 2. The drug according to claim 1, where the cells contain two or more HLA alleles from table 4. ! 3. The drug according to claim 1, where the cells contain three or more HLA alleles from table 4. ! 4. The drug according to claim 1, where the cells contain four or more HLA alleles from table 4. ! 5. The drug according to claim 1, where the cells contain all HLA alleles from table 4. ! 6. The drug according to claim 1, where the drug proliferates in culture in vitro for more than one year. ! 7. The formulation of claim 1, wherein cell differentiation is inhibited when the cells are cultured on the fibroblast feeder layer. ! 8. The formulation of claim 7, wherein the fibroblast feeder layer is a human fibroblast feeder layer. ! 9. The formulation of claim 7, wherein the fibroblast feeder layer is a mouse fibroblast feeder layer. ! 10. The preparation according to claim 1, wherein cell differentiation is inhibited when cultivated in an in vitro culture without a feeder. ! 11. The preparation of claim 1, wherein the preparation remains largely undifferentiated after 40 passages in culture. ! 12. The preparation of claim 1, wherein the preparation remains substantially undifferentiated after 100 passages in culture. ! 13. The preparation according to claim 1, wherein the cells additionally contain one or more short tandem repeat loci from Table 5. ! 14. The preparation according to claim 1, where the cells additionally contain two or more |
| priorityDate | 2005-05-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 26.