http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2007146174-A
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P21-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-555 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-63 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-93 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-62 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-86 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-1044 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-54 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-16 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-01 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-63 |
| filingDate | 2006-05-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2009-06-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | RU-2007146174-A |
| titleOfInvention | PROTEIN PRODUCTION METHOD |
| abstract | 1. A method of protein production, comprising culturing an obligate methanol-assimilating bacterium in a liquid medium containing methanol as the main carbon source to ensure the secretion of the target protein by the bacterium, and isolating the secreted target protein, wherein said bacterium has a DNA construct containing a promoter sequence functioning in a methanol-assimilating bacterium; and a nucleotide sequence encoding a polypeptide comprising a signal sequence and a target protein sequence operably linked to a promoter sequence. ! 2. The method of claim 1, wherein the promoter sequence functioning in the methanol-assimilating bacterium is isolated from the group consisting of a methanol dehydrogenase promoter, a tac promoter, a σE promoter and a ribosomal protein promoter. ! 3. The method of claim 1, wherein the promoter sequence is the nucleotide sequence of SEQ ID NO: 11, 12, 21 or 22.! 4. The method of claim 1, wherein the signal sequence is a signal sequence of a protein selected from the group consisting of methanol dehydrogenase, phytase, and acid phosphatase. ! 5. The method of claim 1, wherein the signal sequence has an amino acid sequence selected from SEQ ID NO: 18 and SEQ ID NO: 20.! 6. The method of claim 1, wherein the protein is selected from the group consisting of phytase, interleukin, transglutaminase, interferon, insulin, acid phosphatase, and peptide synthase. ! 7. A method according to claims 1-6, wherein the obligate methanol assimilating bacterium is selected from the group consisting of bacteria belonging to the genera Methylophilus, Methylobacillus and Methylophaga. |
| priorityDate | 2005-05-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 118.