http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2007143021-A
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-50 |
| filingDate | 2007-11-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2009-05-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | RU-2007143021-A |
| titleOfInvention | TSITOBIOHIMICHESKY METHOD FOR DETERMINING succinate dehydrogenase activity, oxidation of endogenous succinic acid, ALARM ACTION micromolar concentrations of succinic acid, its use for quantitative estimate of an adrenergic REGULATION IN THE BODY, ENVIRONMENT AND KIT FOR CARRYING OUT THE METHOD |
| abstract | 1. The cytobiochemical method for determining the activity of succinate dehydrogenase (SDH) in combination with the determination of the oxidation of endogenous succinic acid (JNT) and the signaling effect of micromolar concentrations of JNT by measuring on a blood smear the recovery of nitrosine tetrazolium (HCT) in lymphocytes after incubation in an aqueous medium containing KCl 100-150 mm, mainly 125 mm; HEPES 5-15 mM, preferably 10 mM; HCT 0.8-1.5 mm, mainly 1.22 mm; pH 7.2 ± 0.05; JANT 5 μm-5 mm, mainly 5 mm; malonic acid (MAL) 1-5 mm, mainly 5 mm; and 1-Isolimonic Acid (IFL) 50 μM-5 mM, preferably 5 mM; and, if necessary, other substrates and regulators of mitochondrial processes. ! 2. The method according to claim 1, in which the concentration of succinic acid is equal to 5 mm or 5 μm, malonate 5 mm, isolimonic acid 5 mm. ! 3. A quantitative method for determining the level of adrenergic regulation in the body by measuring the value of the LDH activity index by the method according to claim 1 or 2 and calculating, including the subtraction of the HCT recovery index by endogenous substrates (ES) without added YNT in the presence of MAL, if its addition reduces the reduction, or only ES without MAL, if MAL increases restoration, assuming that in a state of physiological deep rest without activation by adrenaline, there is a complete absence of LDH activity (latin state dormant or dormant LDH), an increase in the activity of LDH reflects first the physiological activation of adrenergic regulation, then the pathogenic hyperactivation and pathological inhibition, which are detected by the addition of ELR: the absence of the influence of ELR on the activity of LDH is characteristic of |
| priorityDate | 2007-11-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 31.