http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2007141705-A
Outgoing Links
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|---|---|
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| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N7-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-86 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P31-12 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P31-16 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K39-12 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K39-145 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0606 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0609 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K39-00 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-0735 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K39-12 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N7-00 |
| filingDate | 2006-04-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2009-05-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | RU-2007141705-A |
| titleOfInvention | METHOD FOR PRODUCING ANTIVIRAL VACCINE IN SUSPENSION LINES OF ANIMAL EMBRYONIC STEM CELLS |
| abstract | 1. The method of replication of a virus that is selected from the group consisting of adenoviruses, hepatadaviruses, herpes viruses, orthomyxoviruses, papovaviruses, paramyxoviruses, picornaviruses, poxviruses, reoviruses and retroviruses, in the bird embryonic stem cells of the ЕВх® stage, including: a) the proliferation of these EBx cells in culture vessels, in suspension, in serum-free medium No. 1; ! b) infection of said cells with a selected virus at a cell density of at least about 1.5 million cells / ml; ! c) adding serum-free medium No. 2 to the cell culture shortly before infection, simultaneously with infection or immediately after infection; and! d) further culturing said infected cells in order to create conditions for virus replication; and! e) optionally collecting said virus. ! 2. The method according to claim 1, whereby a continuously stirred bioreactor tank is used as a culture vessel. ! 3. The method according to claim 1, whereby serum-free medium No. 1 and serum-free medium No. 2 are one and the same medium. ! 4. The method according to claim 1, according to which serum-free medium No. 1 and serum-free medium No. 2 have a different composition. ! 5. The method according to claim 1, according to which from 0.25 to 10 volumes of serum-free medium No. 2 was added to serum-free medium No. 1. ! 6. The method according to claim 1, whereby serum-free medium No. 1 and / or serum-free medium No. 2 is supplemented with at least one ingredient selected from the group consisting of amino acids, lipids, fatty acids, cholesterol, carbohydrates, protein hydrolysates |
| priorityDate | 2005-04-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 84.